In vitro determination of the antioxidant capacity of extracts and phenolic compounds from Ugni molinae Turcz. leaves

Resumen

Introduction: Ugni molinae Turcz., (Myrtaceae) is a plant that is distributed in central-southern of Chile, including Juan Fernández Islands. The leaves have been used in folk medicine for diarrhea and oral infections due to their astringent and antiseptic properties.
Objective: antioxidant activity of two extracts (ethyl acetate and methanol) from U. molinae leaves was assessed in several assays.
Methods: HPLC (high performance liquid chromatography) for chemical analysis and in vitro antioxidant methods.
Results: HPLC profiling of both extracts showed the presence of flavan-3-ols (catechin) and gallic acid, flavonoids and caffeic acid derivates as main constituents. These extracts showed significant activity on 2,2-diphenyl-1-picrylhydrazyl (DPPH), cupric ion reducing antioxidant capacity (CUPRAC) and hydroxyl radical assays. It is noteworthy that bleaching of b-carotene-linoleate liposomes and copper-induced oxidation of human LDL were prevented by both extracts.
Conclusions: results suggest that polyphenol-rich extracts of U. molinae could slow down lipid peroxidation and limit free radical damage.